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KMID : 0043320070300050659
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Archives of Pharmacal Research
2007 Volume.30 No. 5 p.659 ~ p.664
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Comparison of Several Polysaccharide-derived Chiral Stationary Phases for the Enantiomer Separation of N-Fluorenylmethoxycarbonyl α-Amino Acids by HPLC
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Jin Jing Yu
Lee Kyung-Ah
Kang Jong-Seong
Kang Young-Koo
Baek Chae-Sun
Lee Won-Jae
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Abstract
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The liquid chromatographic enantiomer separation of N-fluorenylmethoxycarbonyl (FMOC) protected α-amino acids was performed on nine polysaccharide-derived chiral stationary phases (CSPs). The cellulose derived coated CSPs, Chiralcel OD-H (separation factor = 1.09- 2.70) and Chiralcel OD (separation factor = 1.08-2.55), had the best performance of all the CSPs for resolution of N-FMOC α-amino acids and therefore, all analyte enantiomers were base-line separated on Chiralcel OD-H and/or Chiralcel OD. Enantioseparation on cellulose tris(3,5-dimethylphenylcarbamate) derived CSPs (Chiralcel OD-H, Chiralcel OD and Chiralpak IB) is generally greater than that on amylose tris(3,5-dimethylphenylcarbamate) derived CSPs (Chiralpak AD-RH, Chiralpak AD and Chiralpak IA). Additionally, coated type CSPs (Chiralcel OD-H or Chiralcel OD, and Chiralpak AD) generally provided better enantioseparation for these analytes than the covalently bonded type CSPs (Chiralpak IB and Chiralpak IA) with the same chiral selector of cellulose tris(3,5-dimethylphenylcarbamate) and amylose tris(3,5-dimethylphenylcarbamate),respectively. However, Chiralpak IB and Chiralpak IA had an advantage over the coated type CSPs in that a broader range of solvents could be used due to its covalently bonded nature.
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KEYWORD
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Chiral stationary phase, Enantiomer separation, N-Fluorenylmethoxy-carbonyl α-amino acids
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